Immunofluorescence is the immunoassay technique that uses a detector antibody or an antigen labeled with florescent dyes.
Immunofluorescence (IF) is a common morphological approach used to determine the distribution of subcellular components. Antibodies that conjugated with fluorescent dyes are required in IF assay. The antibody specifically recognizes the antigen by binding to the epitope of target, and the fluorophore will be detected under a fluorescent microscope. Hence, subcellular components can be visualized in a dark background. IF can also be used as an alternative semiquantitative analysis method to monitoring the expression of the interest.
Immunofluorescence Test Types
SARS-CoV-2 Spike Protein Antibodies Test Kit (Immunofluorescence)
SARS-CoV-2 Neutralizing Antibody Test Kit (Immunofluorescence)
What are the Advantages of Direct Immunofluorescence Antibody Test？
Clear positioning. This assay can give research on the clear subcellular localization of molecules. The expression of molecules can be observed directly.
High specificity. The preparation of the sample can effectively protect the natural structure of the antigen
High sensitivity. The target with low expression can be detected with immunofluorescence.
Easy to operate. The test result can get quickly. This assay can be used for in-vivo experiments.
Multiple staining. The multiple targets can be detected in the same sample.
Immunofluorescence Test FAQs
Q
What is immunofluorescence and how does it work?
Immunofluorescence is an assay which is used primarily on biological samples and is classically defined as a procedure to detect antigens in cellular contexts using antibodies. The specificity of antibodies to their antigen is the base for immunofluorescence.
Q
What are fluorescent antibody tests?
Q
What is direct fluorescent antibody tests？
Q
What is indirect fluorescent antibody test？
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